reporter gene prl-null (Promega)
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Reporter Gene Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reporter+gene+prl-null/reporter+plasmid+prlnull/pmc03810276-123-1-9
Average 90 stars, based on 1 article reviews
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1) Product Images from "Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress"
Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Journal: PLoS ONE
doi: 10.1371/journal.pone.0077874
Figure Legend Snippet: Primers, strains, and plasmids used in the study.
Techniques Used: Gene Knockout, Construct, Knock-Out, Transduction, Plasmid Preparation, Luciferase, Clone Assay
Figure Legend Snippet: Panel A: Luciferase reporter activity of S. aureus 8325-4 geh ::pLH75 ( Rluc without a promoter, black bars), S. aureus 8325-4 geh ::pLH76 (P mntABC - Rluc , gray bars), and S. aureus 8325-4 mntR :: ermC geh ::pLH76, (P mntABC - Rluc with mntR deleted, white bars) in the presence of increasing Mn 2+ as revealed by luminescence from the Rluc reporter. Panel B: mntABC promoter activity of 8325-4 geh ::pLH76 in the presence of two-fold serial dilutions of Mn 2+ as revealed by luminescence from the Rluc reporter. Error bars indicate one standard deviation from the mean of triplicate wells.
Techniques Used: Luciferase, Activity Assay, Standard Deviation
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Amplification:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Gene Knockout:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Construct:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Knock-Out:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Transduction:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Plasmid Preparation:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Luciferase:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Clone Assay:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Activity Assay:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from Standard Deviation:Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress Article Snippet: The promoterless Rluc reporter gene was amplified from |