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Promega reporter gene prl-null
Primers, strains, and plasmids used in the study.
Reporter Gene Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reporter+gene+prl-null/reporter+plasmid+prlnull/pmc03810276-123-1-9
Average 90 stars, based on 1 article reviews
reporter gene prl-null - by Bioz Stars, 2026-09
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1) Product Images from "Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress"

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress

Journal: PLoS ONE

doi: 10.1371/journal.pone.0077874

Primers, strains, and plasmids used in the study.
Figure Legend Snippet: Primers, strains, and plasmids used in the study.

Techniques Used: Gene Knockout, Construct, Knock-Out, Transduction, Plasmid Preparation, Luciferase, Clone Assay

Panel A: Luciferase reporter activity of S. aureus 8325-4 geh ::pLH75 ( Rluc without a promoter, black bars), S. aureus 8325-4 geh ::pLH76 (P mntABC - Rluc , gray bars), and S. aureus 8325-4 mntR :: ermC geh ::pLH76, (P mntABC - Rluc with mntR deleted, white bars) in the presence of increasing Mn 2+ as revealed by luminescence from the Rluc reporter. Panel B: mntABC promoter activity of 8325-4 geh ::pLH76 in the presence of two-fold serial dilutions of Mn 2+ as revealed by luminescence from the Rluc reporter. Error bars indicate one standard deviation from the mean of triplicate wells.
Figure Legend Snippet: Panel A: Luciferase reporter activity of S. aureus 8325-4 geh ::pLH75 ( Rluc without a promoter, black bars), S. aureus 8325-4 geh ::pLH76 (P mntABC - Rluc , gray bars), and S. aureus 8325-4 mntR :: ermC geh ::pLH76, (P mntABC - Rluc with mntR deleted, white bars) in the presence of increasing Mn 2+ as revealed by luminescence from the Rluc reporter. Panel B: mntABC promoter activity of 8325-4 geh ::pLH76 in the presence of two-fold serial dilutions of Mn 2+ as revealed by luminescence from the Rluc reporter. Error bars indicate one standard deviation from the mean of triplicate wells.

Techniques Used: Luciferase, Activity Assay, Standard Deviation

Related Articles

Amplification:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Gene Knockout:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Construct:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Knock-Out:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Transduction:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Plasmid Preparation:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Luciferase:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Clone Assay:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Activity Assay:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Standard Deviation:

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress
Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.



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Image Search Results


Primers, strains, and plasmids used in the study.

Journal: PLoS ONE

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress

doi: 10.1371/journal.pone.0077874

Figure Lengend Snippet: Primers, strains, and plasmids used in the study.

Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Techniques: Gene Knockout, Construct, Knock-Out, Transduction, Plasmid Preparation, Luciferase, Clone Assay

Panel A: Luciferase reporter activity of S. aureus 8325-4 geh ::pLH75 ( Rluc without a promoter, black bars), S. aureus 8325-4 geh ::pLH76 (P mntABC - Rluc , gray bars), and S. aureus 8325-4 mntR :: ermC geh ::pLH76, (P mntABC - Rluc with mntR deleted, white bars) in the presence of increasing Mn 2+ as revealed by luminescence from the Rluc reporter. Panel B: mntABC promoter activity of 8325-4 geh ::pLH76 in the presence of two-fold serial dilutions of Mn 2+ as revealed by luminescence from the Rluc reporter. Error bars indicate one standard deviation from the mean of triplicate wells.

Journal: PLoS ONE

Article Title: Regulation of Staphylococcus aureus MntC Expression and Its Role in Response to Oxidative Stress

doi: 10.1371/journal.pone.0077874

Figure Lengend Snippet: Panel A: Luciferase reporter activity of S. aureus 8325-4 geh ::pLH75 ( Rluc without a promoter, black bars), S. aureus 8325-4 geh ::pLH76 (P mntABC - Rluc , gray bars), and S. aureus 8325-4 mntR :: ermC geh ::pLH76, (P mntABC - Rluc with mntR deleted, white bars) in the presence of increasing Mn 2+ as revealed by luminescence from the Rluc reporter. Panel B: mntABC promoter activity of 8325-4 geh ::pLH76 in the presence of two-fold serial dilutions of Mn 2+ as revealed by luminescence from the Rluc reporter. Error bars indicate one standard deviation from the mean of triplicate wells.

Article Snippet: The promoterless Rluc reporter gene was amplified from pRL-null (Promega, Madison, WI) with primers oLH434 and oLH435.

Techniques: Luciferase, Activity Assay, Standard Deviation